THIOL GROUPS IN DEOXYRIBONUCLEIC ACID NUCLEOTIDYLTRANSFERASE
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چکیده
منابع مشابه
Thiol Groups in Deoxyribonucleic Acid Nucleotidyltransferase.
1. The inhibitory effects of iodoacetate, iodoacetamide, p-hydroxymercuribenzoate and sarkomycin were studied in a partially purified preparation of deoxyribonucleic acid nucleotidyltransferase from Landschutz ascites-tumour cells. All of these agents inhibited the activity of the enzyme, and it was shown that the inhibition exerted by the last three compounds obeyed non-competitive kinetics. 2...
متن کاملStimulation and Inhibition of Deoxyribonucleic Acid Nucleotidyltransferase
The importance of the physicochemical condition of primer DNA in the DNA nucleotidyltransferase (polymerase) system (deoxynucleoside triphosphate-DNA deoxynucleotidyltransferase, EC 2.7.7.7) from mouse Landschutz ascitestumour cells has been emphasized by Keir, Binnie & Smellie (1962). The ability of DNA to prime the reaction was enhanced by 60% after limited pretreatment with pancreatic deoxyr...
متن کاملThe inhibition of deoxyribonucleic acid nucleotidyltransferase by stilboestrol derivatives.
The inhibition by diethylstilboestrol of DNA nucleotidyltransferase isolated from calf thymus was studied. The inhibition exercised by diethylstilboestrol appears to obey competitive kinetics with respect to DNA primer. The activities of both replicative and terminal enzymes were affected to the same extent. There was no evidence of binding between DNA and diethylstilboestrol. A comparative stu...
متن کاملDeoxyribonucleic acid nucleotidyltransferase from Landschütz ascites-tumour cells.
1. A purification procedure for DNA nucleotidyltransferase from Landschütz ascites-tumour cells is described. The enzyme can be separated from endogenous nucleic acid and from triphosphatase and deoxyribonuclease activities measurable at pH7.5. 2. The basic properties of the nucleotidyltransferase reaction are as follows. The enzyme has optimum activity at pH7.2-7.4. It displays an absolute req...
متن کاملStudies on the synthesis of deoxyribonucleic acid by mammalian enzymes. II. An investigation of the primer requirements of partially purified regenerating rat liver deoxyribonucleic acid nucleotidyltransferase.
A study has been made of the deoxyribonucleic acid primer requirements of partially purified deoxyribonucleic acid deoxynucleotidyltransferase fractionated from regenerating liver of the adult rat. Native, double stranded DNAs derived from either viral, bacterial, or mammalian sources serve more effectively as primers than either single stranded or heat-denatured DNAs for the incorporation of d...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1965
ISSN: 0006-2936
DOI: 10.1042/bj0950483